Comparison and Validation of IS900-Sequence andPutative Sequences as a Specific Target to DetectMycobacterium avium subsp. Paratuberculosis
نویسندگان
چکیده
1Department of Pathobiology, College of Veterinary Medicine, Nursing & Allied Health, Tuskegee University, Analysis, Tuskegee, Alabama, USA 2Pharmaceutical Sciences, Mercer University Atlanta Campus, Atlanta, Georgia, USA 3Thompson-Bishop-Sparks State Diagnostic Laboratory, Auburn, Alabama, USA 4Department of Animal Sciences, Auburn University, Auburn, Alabama, USA 5Center for Computational Epidemiology, Bioinformatics & Risk, College of Veterinary Medicine, Nursing & Allied Health, Tuskegee University, Analysis Tuskegee, Alabama, USA Received date: 25 May 2017; Accepted date: 17 Jul 2017; Published date: 24 Jul 2017.
منابع مشابه
Detection of Mycobacterium avium subsp. paratuberculosis in the mesenteric lymph nodes of goats by PCR and culture
The efficacy of bacterial cultures and IS900-specific polymerase chain reaction (PCR) was compared for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) from the mesenteric lymph nodes of goats. Samples were collected from 75 goats slaughtered in Ilam, in southwest of Iran. Tissue homogenates were inoculated onto four media. The genomic DNA was extracted directly from mesenteri...
متن کاملWhat is Mycobacterium avium subsp. paratuberculosis?
We read with interest the article by Beumer et al. (1) on the detection of Mycobacterium avium subsp. paratuberculosis in 81% of drinking water and biofilm samples by PCR. Although these findings could have a significant influence on our understanding and the potential public impact of M. avium subsp. paratuberculosis as an agent of disease, much of the information on M. avium subsp. paratuberc...
متن کاملDirect detection of Mycobacterium avium subsp. Paratuberculosis in bovine milk by multiplex Real-time PCR
The study aimed at direct detection of Mycobacterium avium subsp. Paratuberculosis (MAP) in milk by evaluating a multiplex real-time PCR assay targeting IS900 and ISMAV2 sequences including the amplification of PUC19-plasmid as internal control. The sensitivity of the assays was evaluated by testing MAP isolates in broad linear range of DNA (50 ng – 5 fg/μl). For the validation of the specifici...
متن کاملCharacterization of IS900 loci in mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing
Mycobacterium avium subsp. paratuberculosis is a pathogen that causes chronic inflammation of the intestine in many animals, including primates, and is implicated in Crohn's disease in humans. It differs from other members of the M. avium complex in having 14-18 copies of IS900 inserted into conserved loci in its genome. In the present study, genomic DNA flanking 14 of these insertions was char...
متن کاملDetection and verification of Mycobacterium avium subsp. paratuberculosis in fresh ileocolonic mucosal biopsy specimens from individuals with and without Crohn's disease.
Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp. paratuberculosis infection is widespread in domestic livestock and is present in retail pasteurized cows' milk in the United Kingdom and, potentially, elsewhere. Water supplies are also at risk. The ...
متن کامل